Demonstration data. Every lot code, purity figure, chromatogram, certificate, laboratory and date on this site is a sample for design review. None of it is a real measurement.
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How do I read a chromatogram, and does 99% purity mean the vial contains 99% of its labeled amount?

A chromatogram shows peaks over time; retention time identifies a substance and peak area indicates relative amount. Purity is not the same measurement as fill content.

A chromatogram is the output of a chromatography run: a graph of detector signal against time, showing one or more peaks as different substances in the sample exit the column. Retention time is how long a substance takes to travel through the column and reach the detector — a consistent property of a given substance under the same method conditions, used to help identify what a peak represents. The relative area under each peak is generally used to calculate purity as a percentage of everything detected.

A purity percentage is a statement about chemical purity — how much of what was detected in the sample is the intended compound, relative to other detected substances — not a statement about how much material is physically in the vial. A 99% purity result does not, by itself, mean the vial contains 99% of its printed milligram amount; that is a separate question about fill content or assay, which is why identity, purity, and content are reported as distinct results rather than one combined figure.

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